Sample Preparation and Post-separation Analysis

P01009

General information about the entry
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Entry name	A1AT_HUMAN
Primary accession number	P01009
integrated into SWISS-2DPAGE on	August 1, 1993 (release 0)
2D Annotations were last modified on	May 15, 2003 (version 1)
General Annotations were last modified on	January 31, 2008 (version 17)
Name and origin of the protein
Description	Alpha-1-antitrypsin (Alpha-1 protease inhibitor) (Alpha-1-antiproteinase).
Gene name	Name=SERPINA1 Synonyms=AAT, PI ORFNames=PRO0684, PRO2209
Annotated species	Homo sapiens (Human) [TaxID: 9606]
Taxonomy	Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan] Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J. ''Human liver protein map: a reference database established by microsequencing and gel comparison'' Electrophoresis 13:992-1001(1992) [2]   PATHOLOGICAL LEVEL Allen R.C., Bienvenu J., Laurent P., Suskind R.M. (In) Marker proteins in inflammation: proceedings of the symposium, Lyon, France, April 1981, de Gruyter, Berlin (1982) [3]   PATHOLOGICAL VARIANTS Sharp H.L. (IN) McKusick V.A., Clairbone R. (eds.)Medical genetics, pp.131-140, H. P. Publ. Co. Inc. (1973) [4]   GENETIC VARIANTS MEDLINE=80181048; PubMed=6102963; [NCBI, ExPASy, EBI, Israel, Japan] Cox D.W., Johnson A.M., Fagerhol M.K. ''Report of Nomenclature Meeting for alpha 1-antitrypsin, INSERM, Rouen/Bois-Guillaume-1978'' Hum. Genet. 53:429-433(1980) [5]   PATHOLOGICAL VARIANTS MEDLINE=83297520; PubMed=6604220; [NCBI, ExPASy, EBI, Israel, Japan] Owen M.C., Brennan S.O., Lewis J.H., Carrell R.W. ''Mutation of antitrypsin to antithrombin. alpha 1-antitrypsin Pittsburgh (358 Met leads to Arg), a fatal bleeding disorder'' New Engl. J. Med. 309:694-698(1983) [6]   MAPPING ON GEL MEDLINE=93092937; PubMed=1459097; [NCBI, ExPASy, EBI, Israel, Japan] Hughes G.J., Frutiger S., Paquet N., Ravier F., Pasquali C., Sanchez J.-C., James R.W., Tissot J.-D., Bjellqvist B., Hochstrasser D.F. ''Plasma protein map: an update by microsequencing'' Electrophoresis 13:707-714(1992) [7]   MAPPING ON GEL MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan] Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F. ''Inside SWISS-2DPAGE database'' Electrophoresis 16:1131-1151(1995)
Comments
SUBUNIT: SINGLE CHAIN
2D PAGE maps for identified proteins
How to interpret a protein
CSF_HUMAN {Cerebrospinal Fluid} Homo sapiens (Human) Tissue: Cerebrospinal fluid   map experimental info   protein estimated location		CSF_HUMAN MAP LOCATIONS: SPOT 2D-000BG6: pI=4.94; Mw=132121 SPOT 2D-000BGA: pI=5.00; Mw=132770 SPOT 2D-000BGH: pI=5.06; Mw=130832 SPOT 2D-000BTP: pI=4.78; Mw=60493 SPOT 2D-000BTU: pI=4.83; Mw=59122 SPOT 2D-000BU8: pI=4.90; Mw=57782 SPOT 2D-000BUC: pI=4.79; Mw=58523 SPOT 2D-000BUV: pI=4.96; Mw=57051 SPOT 2D-000BV7: pI=5.04; Mw=56473 SPOT 2D-000BVN: pI=5.08; Mw=56043 SPOT 2D-000BVO: pI=5.12; Mw=57051 SPOT 2D-000BWW: pI=5.15; Mw=55193 MAPPING (identification): MATCHING WITH THE PLASMA MASTER GEL [7].
HEPG2SP_HUMAN {HepG2 Secreted Proteins} Homo sapiens (Human) Tissue: Hepatoblastoma   map experimental info   protein estimated location		HEPG2SP_HUMAN MAP LOCATIONS: SPOT 2D-0008W1: pI=4.62; Mw=57821 SPOT 2D-0008WN: pI=4.69; Mw=55230 SPOT 2D-0008WS: pI=4.74; Mw=54511 SPOT 2D-0008WZ: pI=4.80; Mw=53979 SPOT 2D-0008YD: pI=4.87; Mw=52240 SPOT 2D-0008YS: pI=4.95; Mw=51391 SPOT 2D-0008YY: pI=5.04; Mw=51223 SPOT 2D-0008Z4: pI=5.12; Mw=51223 MAPPING (identification): MATCHING WITH THE PLASMA MASTER GEL [7].
LIVER_HUMAN {Liver} Homo sapiens (Human) Tissue: Liver   map experimental info   protein estimated location		LIVER_HUMAN MAP LOCATIONS: SPOT 2D-0000LY: pI=4.92; Mw=56382 SPOT 2D-0000MB: pI=4.97; Mw=55609 SPOT 2D-0000MX: pI=5.01; Mw=54544 SPOT 2D-0000NT: pI=5.08; Mw=53944 DISEASE POSITIONAL VARIANTS: ALPHA-1-ANTITRYPSIN PITTSBURGH [5]. NORMAL POSITIONAL VARIANTS: 30 GENETIC VARIANTS KNOWN AS PI ALLELES [4]. PATHOLOGICAL LEVEL: DECREASED DURING HEPATIC CIRRHOSIS IN CHILDREN [3] AND EMPHYSEMA; INCREASED DURING THE ACUTE-PHASE REACTION [2], LIVER DAMAGE AND PREGNANCY. MAPPING (identification): MATCHING WITH A PLASMA GEL [1].
LYMPHOMA_HUMAN {Lymphoma} Homo sapiens (Human) Tissue: Lymphoma   map experimental info   protein estimated location		LYMPHOMA_HUMAN MAP LOCATIONS: SPOT 2D-0007GG: pI=4.87; Mw=55663 SPOT 2D-0007H1: pI=4.92; Mw=54763 SPOT 2D-0007HH: pI=5.00; Mw=53878 SPOT 2D-0007HT: pI=5.08; Mw=53503 MAPPING (identification): MATCHING WITH THE LIVER MASTER GEL [7].
PLASMA_HUMAN {Plasma} Homo sapiens (Human) Tissue: Plasma   map experimental info   protein estimated location		PLASMA_HUMAN MAP LOCATIONS: SPOT 2D-0004B2: pI=5.01; Mw=108882 SPOT 2D-0004B8: pI=5.05; Mw=106590 SPOT 2D-0004KJ: pI=5.11; Mw=69340 SPOT 2D-0004QH: pI=4.91; Mw=57706 SPOT 2D-0004QK: pI=4.87; Mw=57549 SPOT 2D-0004QS: pI=4.95; Mw=56773 SPOT 2D-0004R9: pI=5.00; Mw=56313 SPOT 2D-0004RF: pI=4.91; Mw=56466 SPOT 2D-0004RM: pI=4.87; Mw=56313 SPOT 2D-0004RW: pI=5.05; Mw=55856 SPOT 2D-0004SF: pI=4.91; Mw=55252 SPOT 2D-0004SO: pI=4.95; Mw=54953 SPOT 2D-0004SQ: pI=5.00; Mw=54953 SPOT 2D-0004SZ: pI=5.05; Mw=54507 SPOT 2D-0004TO: pI=4.95; Mw=54065 SPOT 2D-0004TP: pI=4.99; Mw=53626 SPOT 2D-0004TU: pI=5.10; Mw=53626 SPOT 2D-0004TW: pI=5.04; Mw=53336 SPOT 2D-0004WJ: pI=4.87; Mw=50380 SPOT 2D-0004WZ: pI=4.91; Mw=49297 SPOT 2D-0004X0: pI=4.95; Mw=49431 SPOT 2D-0004XH: pI=4.99; Mw=48369 NORMAL LEVEL: 1900-3500 MG/L. PATHOLOGICAL LEVEL: DECREASED DURING HEPATIC CIRRHOSIS IN CHILDREN [3] AND EMPHYSEMA; INCREASED DURING THE ACUTE-PHASE REACTION [2], LIVER DAMAGE AND PREGNANCY. POSITIONAL VARIANTS: 30 GENETIC VARIANTS KNOWN AS PI ALLELES [4]. MAPPING (identification): MICROSEQUENCING [6].
Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://www.expasy.org/ch2d/license.html or send email to license@isb-sib.ch).
Cross-references
DOSAC-COBS-2DPAGE	P01009; A1AT_HUMAN.
HSC-2DPAGE	P01009; HUMAN.
OGP-WWW	P01009.
REPRODUCTION-2DPAGE	IPI00553177; A1AT_HUMAN.
REPRODUCTION-2DPAGE	P01009; P01009.
Siena-2DPAGE	P01009; A1AT_HUMAN.
UniProtKB/Swiss-Prot	P01009; A1AT_HUMAN.
2D PAGE maps for unidentified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
Arabidopsis thaliana (Mouse-ear cress)	Arabidopsis thaliana
Dictyostelium discoideum (Slime mold)	Dictyostelium discoideum
Escherichia coli	Escherichia coli Escherichia coli DIGE (4.5-6.5) Escherichia coli(4-5) Escherichia coli(4.5-5.5) Escherichia coli(5-6) Escherichia coli(5.5-6.7) Escherichia coli(6-11) Escherichia coli(6-9)
Homo sapiens (Human)	Colorectal epithelia cells Colorectal adenocarcinoma cell line (DL-1) Erythroleukemia Cell HepG2 Promyelocytic leukemia cells Kidney Lymphocytes Soluble nuclear proteins and matrix from liver tissue SDS-PAGE of nucleolar proteins from Human HeLa cells 2D-PAGE of nucleolar proteins from Human HeLa cells Platelet Red blood cells Macrophage Like Cell Line
Mus musculus (Mouse)	Brown adipose tissue Pancreatic islet cells Liver Gastrocnemius muscle Soluble nuclear proteins and matrix from liver tissue White adipose tissue
Saccharomyces cerevisiae (Baker's yeast)	Saccharomyces cerevisiae
Staphylococcus aureus (strain N315)	Staphylococcus aureus