NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 36-50.
STRAIN=ATCC 621 / IFO 3172 / NCIB 7069;
PubMed=9055427 [NCBI, ExPASy, EBI, Israel, Japan]
Kondo K., Horinouchi S.;
"Characterization of the genes encoding the three-component membrane-bound alcohol dehydrogenase from Gluconobacter suboxydans and their expression in Acetobacter pasteurianus.";
Appl. Environ. Microbiol. 63:1131-1138(1997).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=621H;
DOI=10.1038/nbt1062; PubMed=15665824 [NCBI, ExPASy, EBI, Israel, Japan]
Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U.;
"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans.";
Nat. Biotechnol. 23:195-200(2005).

Comments

FUNCTION: Dehydration of primary alcohols (exception: methanol) (By similarity).
CATALYTIC ACTIVITY: A primary alcohol + acceptor = an aldehyde + reduced acceptor.
COFACTOR: Binds 1 PQQ group per subunit. PQQ is inserted between disulfide Cys-141-Cys-142 and the indole ring of Trp-279 (By similarity).
COFACTOR: Binds 1 calcium ion per subunit (By similarity).
COFACTOR: Binds 1 heme group per subunit (By similarity).
SUBUNIT: Heterotrimer (dehydrogenase, cytochrome and protein adhS), that forms the alcohol dehydrogenase complex.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Periplasmic side (Potential).
SIMILARITY: Belongs to the bacterial PQQ dehydrogenase family.
SIMILARITY: Contains 1 cytochrome c domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D86375; BAA19753.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
CP000009; AAW60837.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_191493.1; -.

3D structure databases

HSSP

Q46444; 1KB0. [HSSP ENTRY / PDB]

ModBase

O05542.

Enzyme and pathway databases

BioCyc

GOXY290633:GOX1068-MON; -.

Ontologies

GO:0030288;	Cellular component: outer membrane-bounded periplasmic space (inferred from electronic annotation from InterPro).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0018468;	Molecular function: alcohol dehydrogenase (acceptor) activity (inferred from electronic annotation from EC).
GO:0005509;	Molecular function: calcium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0020037;	Molecular function: heme binding (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR009056; Cyt_c_monohaem.
IPR017512; PQQ_MeOH/EtOH_DH.
IPR002372; PQQ_repeat.
IPR011047; Quino_AlcDHase-like.
IPR001479; Quinoprotein_DHase_PQQ_bac_CS.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.760.10; Cytochrome_c_R; 1.
G3DSA:2.140.10.10; Quinoprotein_alc_DH-like; 1.

Pfam

PF01011; PQQ; 6.
Pfam graphical view of domain structure.

SMART

SM00564; PQQ; 6.
SMART graphical view of domain structure.

PROSITE

PS00363; BACTERIAL_PQQ_1; 1.
PS00364; BACTERIAL_PQQ_2; 1.
PS51007; CYTC; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

GeneID

3250283; -.

GenomeReviews

CP000009_GR; GOX1068.

KEGG

gox:GOX1068; -.

NMPDR

fig|290633.1.peg.1038; -.

Phylogenomic databases

HOGENOM

O05542; -.

Genome annotation databases

CMR

O05542; GOX1068.

Other

ProtoNet

O05542.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Cell membrane; Complete proteome; Direct protein sequencing; Heme; Iron; Membrane; Metal-binding; Oxidoreductase; PQQ; Pyrrolidone carboxylic acid; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 34`	`34`
`CHAIN`	`35 757`	`723`	`Alcohol dehydrogenase [acceptor].`	`PRO_0000025562`
`DOMAIN`	`637 726`	`90`	`Cytochrome c.`
`ACT_SITE`	`342 342`		`Proton acceptor (Potential).`
`METAL`	`215 215`		`Calcium (By similarity).`
`METAL`	`297 297`		`Calcium (By similarity).`
`METAL`	`657 657`		`Iron (heme axial ligand) (By similarity).`
`BINDING`	`653 653`		`Heme (covalent) (By similarity).`
`BINDING`	`656 656`		`Heme (covalent) (By similarity).`
`MOD_RES`	`35 35`		`Pyrrolidone carboxylic acid.`
`DISULFID`	`141 142`		`By similarity.`
`CONFLICT`	`230 230`		`G -> S (in Ref. 1; BAA19753).`
`CONFLICT`	`675 675`		`A -> R (in Ref. 1; BAA19753).`

Sequence information

Length: 757 AA [This is the length of the unprocessed precursor]

Molecular weight: 82853 Da [This is the MW of the unprocessed precursor]

CRC64: 56B3940B711BB0CC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTSGLLTPIK VTKKRLLSCA AALAFSAAVP VAFAQEDTGT AITSSDNGGH PGDWLSYGRS 

        70         80         90        100        110        120 
YSEQRYSPLD QINTENVGKL KLAWHYDLDT NRGQEGTPLI VNGVMYATTN WSKMKALDAA 

       130        140        150        160        170        180 
TGKLLWSYDP KVPGNIADRG CCDTVSRGAA YWNGKVYFGT FDGRLIALDA KTGKLVWSVY 

       190        200        210        220        230        240 
TIPKEAQLGH QRSYTVDGAP RIAKGKVLIG NGGAEFGARG FVSAFDAETG KLDWRFFTVP 

       250        260        270        280        290        300 
NPENKPDGAA SDDILMSKAY PTWGKNGAWK QQGGGGTVWD SLVYDPVTDL VYLGVGNGSP 

       310        320        330        340        350        360 
WNYKFRSEGK GDNLFLGSIV AINPDTGKYV WHFQETPMDE WDYTSVQQIM TLDMPVNGEM 

       370        380        390        400        410        420 
RHVIVHAPKN GFFYIIDAKT GKFITGKPYT YENWANGLDP VTGRPNYVPD ALWTLTGKPW 

       430        440        450        460        470        480 
LGIPGELGGH NFAAMAYSPK TKLVYIPAQQ IPLLYDGQKG GFKAYHDAWN LGLDMNKIGL 

       490        500        510        520        530        540 
FDDNDPEHVA AKKDFLKVLK GWTVAWDPEK MAPAFTINHK GPWNGGLLAT AGNVIFQGLA 

       550        560        570        580        590        600 
NGEFHAYDAT NGNDLYSFPA QSAIIAPPVT YTANGKQYVA VEVGWGGIYP FLYGGVARTS 

       610        620        630        640        650        660 
GWTVNHSRVI AFSLDGKDSL PPKNELGFTP VKPVPTYDEA RQKDGYFMYQ TFCSACHGDN 

       670        680        690        700        710        720 
AISGGVLPDL RWSGAPRGRE SFYKLVGRGA LTAYGMDRFD TSMTPEQIED IRNFIVKRAN 

       730        740        750 
ESYDDEVKAR ENSTGVPNDQ FLNVPQSTAD VPTADHP

O05542 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools