ID   HMDH_METTH              Reviewed;         397 AA.
AC   O26662;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-NOV-2008, entry version 59.
DE   RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase;
DE            Short=HMG-CoA reductase;
DE            EC=1.1.1.34;
GN   Name=hmgA; OrderedLocusNames=MTH_562;
OS   Methanobacterium thermoautotrophicum.
OC   Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC   Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Delta H;
RX   MEDLINE=98037514; PubMed=9371463;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K.,
RA   Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D.,
RA   Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R.,
RA   Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S.,
RA   McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M.,
RA   Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum
RT   deltaH: functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Converts HMG-CoA to mevalonate (By similarity).
CC   -!- CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP(+) = (S)-3-
CC       hydroxy-3-methylglutaryl-CoA + 2 NADPH.
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid
CC       biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
CC   -!- SIMILARITY: Belongs to the HMG-CoA reductase family.
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DR   EMBL; AE000666; AAB85068.1; -; Genomic_DNA.
DR   PIR; D69174; D69174.
DR   RefSeq; NP_275705.1; -.
DR   HSSP; P04035; 1HWI.
DR   GeneID; 1470523; -.
DR   GenomeReviews; AE000666_GR; MTH_562.
DR   KEGG; mth:MTH562; -.
DR   NMPDR; fig|187420.1.peg.560; -.
DR   HOGENOM; O26662; -.
DR   BioCyc; MTHE187420:MTH562-MON; -.
DR   GO; GO:0016021; C:integral to membrane; IEA:InterPro.
DR   GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH)...; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR   GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002202; HMG_CoA_Rdtase_cat.
DR   InterPro; IPR004554; HMG_CoA_Rdtase_I_cat.
DR   Gene3D; G3DSA:3.90.770.10; HMG-CoA_red; 2.
DR   PANTHER; PTHR10572; HMG-CoA_red; 1.
DR   Pfam; PF00368; HMG-CoA_red; 1.
DR   PRINTS; PR00071; HMGCOARDTASE.
DR   TIGRFAMs; TIGR00533; HMG_CoA_R_NADP; 1.
DR   PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR   PROSITE; PS00318; HMG_COA_REDUCTASE_2; FALSE_NEG.
DR   PROSITE; PS01192; HMG_COA_REDUCTASE_3; FALSE_NEG.
DR   PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Isoprene biosynthesis; NADP; Oxidoreductase.
FT   CHAIN         1    397       3-hydroxy-3-methylglutaryl-coenzyme A
FT                                reductase.
FT                                /FTId=PRO_0000114462.
FT   ACT_SITE     96     96       Charge relay system (By similarity).
FT   ACT_SITE    301    301       Charge relay system (By similarity).
FT   ACT_SITE    391    391       Proton donor (By similarity).
SQ   SEQUENCE   397 AA;  42160 MW;  C4AA45A82EF211E4 CRC64;
     MSIMDDLMEG RIKLYEIERH VPVDEAVRIR REFIERTCGV KLEHVSNYSI DMERASRRNI
     ENPIGVVQIP LGVAGPLRVR GEHADGEYYV PLATSEGALV ASVNRGCSVI TRAGGATVRV
     TGDSMTRAPV IRTGSVVEAL QLREWIYENM DALREEAEST TRHGKLVKID PIIVAGSYVY
     PRFVYTTGDS MGMNMVTIAT ERALELLTRE TGAHVIALSG NLCTDKKPAA VNLIEGRGKS
     ITAEITVPGE MVESVLKTTP EAVVEVNTAK NLIGSAAAGS MGFNAHYANI IGAIFLATGQ
     DEAHIVEGSL GVTIAEERKG DLYFAVNLPD VPLATVGGGT GLETASECLD IMGVRGGGRV
     HAFAEIVGGA VLAGELSLMG ALAAGHLARA HSELGRG
//