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UniProtKB/Swiss-Prot entry O74164

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name HMDH_PICJA
Primary accession number O74164
Secondary accession numbers None
Integrated into Swiss-Prot on December 1, 2000
Sequence was last modified on November 1, 1998 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 55)
Name and origin of the protein
Protein name 3-hydroxy-3-methylglutaryl-coenzyme A reductase
Synonyms HMG-CoA reductase
EC 1.1.1.34
Gene name
Name: HMG
From
Pichia jadinii (Yeast) (Candida utilis) [TaxID: 4903] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Pichia.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
PubMed=9647847 [NCBI, ExPASy, EBI, Israel, Japan]
Shimada H., Kondo K., Fraser P.D., Miura Y., Saito T., Misawa N.;
"Increased carotenoid production by the food yeast Candida utilis through metabolic engineering of the isoprenoid pathway.";
Appl. Environ. Microbiol. 64:2676-2680(1998).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB012603; BAA31937.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
3D structure databases
HSSP P04035; 1DQA. [HSSP ENTRY / PDB]
ModBase O74164.
Ontologies
GO
GO:0005789; Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0004420; Molecular function: hydroxymethylglutaryl-CoA reductase (NADPH) activity (inferred from electronic annotation from InterPro).
GO:0050661; Molecular function: NADP binding (inferred from electronic annotation from InterPro).
GO:0006695; Biological process: cholesterol biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0015936; Biological process: coenzyme A metabolic process (inferred from electronic annotation from InterPro).
GO:0008299; Biological process: isoprenoid biosynthetic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR002202; HMG_CoA_Rdtase_cat.
IPR004554; HMG_CoA_Rdtase_I_cat.
IPR000731; SSD_5TM.
Graphical view of domain structure.
Gene3D G3DSA:3.90.770.10; HMG-CoA_red; 1.
PANTHER PTHR10572; HMG-CoA_red; 1.
Pfam PF00368; HMG-CoA_red; 1.
Pfam graphical view of domain structure.
PRINTS PR00071; HMGCOARDTASE.
TIGRFAMs TIGR00533; HMG_CoA_R_NADP; 1.
PROSITE PS00066; HMG_COA_REDUCTASE_1; 1.
PS00318; HMG_COA_REDUCTASE_2; 1.
PS01192; HMG_COA_REDUCTASE_3; 1.
PS50065; HMG_COA_REDUCTASE_4; 1.
PS50156; SSD; 1.
PROSITE graphical view of domain structure (profiles).
Other
ProtoNet O74164.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cholesterol biosynthesis; Endoplasmic reticulum; Glycoprotein; Lipid synthesis; Membrane; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   934  934     3-hydroxy-3-methylglutaryl-coenzyme A reductase. PRO_0000114454
TRANSMEM   112   132  21     Potential. 
TRANSMEM   142   162  21     Potential. 
TRANSMEM   257   277  21     Potential. 
TRANSMEM   335   355  21     Potential. 
TRANSMEM   422   442  21     Potential. 
REGION   443   521  79     Linker. 
REGION   522   934  413     Catalytic. 
ACT_SITE   618   618        Charge relay system (By similarity). 
ACT_SITE   752   752        Charge relay system (By similarity). 
ACT_SITE   828   828        Charge relay system (By similarity). 
ACT_SITE   924   924        Proton donor (By similarity). 
CARBOHYD   361   361        N-linked (GlcNAc...) (Potential). 
CARBOHYD   364   364        N-linked (GlcNAc...) (Potential). 
CARBOHYD   382   382        N-linked (GlcNAc...) (Potential). 
CARBOHYD   682   682        N-linked (GlcNAc...) (Potential). 
Sequence information
Length: 934 AA [This is the length of the unprocessed precursor] Molecular weight: 101234 Da [This is the MW of the unprocessed precursor] CRC64: 3C3E43FC5623601C [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MFYHGASANQ HWIAVDDLSK VPVDVDHYNV VPFQFRRAGE YKEPVLSGIV ELDEVKFVVS 

        70         80         90        100        110        120 
QSDAAEQWQQ LTAEDGTVWR SRAYHGKLGK YSDMAVGAFN KVLNLVRGAE TFDIALVTCA 

       130        140        150        160        170        180 
YIAMFYTLFN LFARMRAVGS KVWLGLSTLV SSFFAFLFAL YITTRVLDLS IPFLSLSEGI 

       190        200        210        220        230        240 
PFFVAVVGFN NKILLAEKVL QNQLNAQSSK NDAPTVLYQA LREQGPLLLR DHLFMITAFL 

       250        260        270        280        290        300 
GCSFYASYLD GLKNFCILAA LILAFDILTT STFLSAILSL KLEINQIHRS TLLREQLEDD 

       310        320        330        340        350        360 
GLTETTVDDV LKSNSLAGTK TFTDAPSTLV TVAKVAGVSV FFGLHFYGFG SAWLSDLSAG 

       370        380        390        400        410        420 
NETNDTFTLY DAVADQIPIG SNGTLVTLFP TRFFLPEKLS TQIEAVVLSF IGLISTAARD 

       430        440        450        460        470        480 
KYISKFILFA FAVSASINVY LLNVARIHTT RLEDAIELKK PKKKASKTAV SVPKAVVVKD 

       490        500        510        520        530        540 
SETTKSSEIL HSSSESESEQ SSRPLEQVIE LYKDGKVKTL VDDEVVSLVT AGKLPLYALE 

       550        560        570        580        590        600 
KQLGDNLRAV AIRRKAISDL ADAPVLRSNK LPYLHYDYDR VFGACCENVI GYMPLPVGVA 

       610        620        630        640        650        660 
GPLIIDGKPY HIPMATTEGC LVASAMRGCK AINLGGGVTT VLTKDGMTRG PCVKFPSLKR 

       670        680        690        700        710        720 
AGQCKLWLDS DEGQEEMKKA FNSTSRFARL QHLQTALAGD LLFIRFRTVT GDAMGMNMIS 

       730        740        750        760        770        780 
KGVEYALKQM TEVFGWDDMM VVSVSGNYCT DKKPAAVNWI NGRGKSVVAE ASIPKDAVVK 

       790        800        810        820        830        840 
VLKSSVKAVV DVNVNKNLIG SAMAGSVGGF NAQAANMVTA VYLALGQDPA QNVESSNCIT 

       850        860        870        880        890        900 
LMTETEDGDL KVSVSMPSIE VGTIGGGTIL DPQGSMLELL GVRGPADVPG ENARQLAKIV 

       910        920        930 
ASIVLSGELS LVSALAAGHL VQSHMQHNRA AAKK
O74164 in FASTA format

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