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UniProtKB/Swiss-Prot entry P09919


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CSF3_HUMAN
Primary accession number P09919
Secondary accession numbers None
Integrated into Swiss-Prot on July 1, 1989
Sequence was last modified on July 1, 1989 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 104)
Name and origin of the protein
Protein name Granulocyte colony-stimulating factor [Precursor]
Synonyms G-CSF
Pluripoietin
Filgrastim
Lenograstim
Gene name
Name: CSF3
Synonyms: GCSF
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1038/319415a0; PubMed=3484805 [NCBI, ExPASy, EBI, Israel, Japan]
Nagata S., Tsuchiya M., Asano S., Kaziro Y., Yamazaki T., Yamamoto O., Hirata Y., Kubota N., Oheda M., Nomura H., Ono M.;
"Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor.";
Nature 319:415-418(1986).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2423327 [NCBI, ExPASy, EBI, Israel, Japan]
Nagata S., Tsuchiya M., Asano S., Yamamoto O., Hirata Y., Kubota N., Oheda M., Nomura H., Yamazaki T.;
"The chromosomal gene structure and two mRNAs for human granulocyte colony-stimulating factor.";
EMBO J. 5:575-581(1986).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=3494801 [NCBI, ExPASy, EBI, Israel, Japan]
Devlin J.J., Devlin P.E., Myambo K., Lilly M.B., Rado T.A., Warren M.K.;
"Expression of granulocyte colony-stimulating factor by human cell lines.";
J. Leukoc. Biol. 41:302-306(1987).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS MET-157 AND THR-174.
SeattleSNPs program for genomic applications;
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [MRNA] OF 19-207.
PubMed=2420009 [NCBI, ExPASy, EBI, Israel, Japan]
Souza L.M., Boone T.C., Gabrilove J., Lai P.H., Zsebo K.M., Murdock D.C., Chazin V.R., Bruszewski J., Lu H., Chen K.K., Barendt J., Platzer E., Moore M.A.S., Mertelsmann R., Welte K.;
"Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells.";
Science 232:61-66(1986).
[6]
PROTEIN SEQUENCE OF 30-46.
DOI=10.1006/abbi.1995.0047; PubMed=8554326 [NCBI, ExPASy, EBI, Israel, Japan]
Haniu M., Horan T., Arakawa T., Le J., Katta V., Rohde M.F.;
"Extracellular domain of granulocyte-colony stimulating factor receptor. Interaction with its ligand and identification of a domain in close proximity of ligand-binding region.";
Arch. Biochem. Biophys. 324:344-356(1995).
[7]
GLYCOSYLATION AT THR-166.
DOI=10.1016/0021-9673(93)83098-D; PubMed=7685769 [NCBI, ExPASy, EBI, Israel, Japan]
Clogston C.L., Hu S., Boone T.C., Lu H.S.;
"Glycosidase digestion, electrophoresis and chromatographic analysis of recombinant human granulocyte colony-stimulating factor glycoforms produced in Chinese hamster ovary cells.";
J. Chromatogr. A 637:55-62(1993).
[8]
STRUCTURE BY NMR.
DOI=10.1016/0014-5793(92)81521-M; PubMed=1281794 [NCBI, ExPASy, EBI, Israel, Japan]
Zink T., Ross A., Ambrosius D., Rudolph R., Holak T.A.;
"Secondary structure of human granulocyte colony-stimulating factor derived from NMR spectroscopy.";
FEBS Lett. 314:435-439(1992).
[9]
STRUCTURE BY NMR.
DOI=10.1021/bi00194a009; PubMed=7518249 [NCBI, ExPASy, EBI, Israel, Japan]
Zink T., Ross A., Luers K., Cieslar C., Rudolph R., Holak T.A.;
"Structure and dynamics of the human granulocyte colony-stimulating factor determined by NMR spectroscopy. Loop mobility in a four-helix-bundle protein.";
Biochemistry 33:8453-8463(1994).
[10]
X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
PubMed=7685117 [NCBI, ExPASy, EBI, Israel, Japan]
Hill C.P., Osslund T.D., Eisenberg D.;
"The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors.";
Proc. Natl. Acad. Sci. U.S.A. 90:5167-5171(1993).
Comments
  • FUNCTION: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • SUBUNIT: Monomer.
  • SUBCELLULAR LOCATION: Secreted.
  • ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
    NameLong
    Isoform IDP09919-1
    This is the isoform sequence displayed in this entry.
    NameShort
    Isoform IDP09919-2
    Features which should be applied to build the isoform sequence: VSP_002673.
  • PTM: O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • PHARMACEUTICAL: Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).
  • SIMILARITY: Belongs to the IL-6 superfamily.
  • CAUTION: PubMed:2420009 misquotes the gene name as "CSF1".
  • WEB RESOURCE: Name=Neupogen/Granulokine; Note=Clinical information on Neupogen/Granulokine; URL="http://www.neupogen.com/";.
  • WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/csf3/";.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X03438; CAA27168.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X03656; CAA27291.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X03655; CAA27290.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M17706; AAA35882.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF388025; AAK62469.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M13008; AAA03056.1; -; Unassigned_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A24573; A24573.
A25093; FQHUGL.
RefSeq NP_000750.1; -.
NP_757373.1; -.
UniGene Hs.2233
3D structure databases
PDB
1CD9; X-ray; 2.80 A; A/C=31-207.[ExPASy / RCSB / EBI]
1GNC; NMR; -; A=-.[ExPASy / RCSB / EBI]
1PGR; X-ray; 3.50 A; A/C/E/G=31-207.[ExPASy / RCSB / EBI]
1RHG; X-ray; 2.20 A; A/B/C=31-207.[ExPASy / RCSB / EBI]
2D9Q; X-ray; 2.80 A; A=31-207.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1CD9; -.
1GNC; -.
1PGR; -.
1RHG; -.
2D9Q; -.
ModBase P09919.
Organism-specific databases
H-InvDB HIX0013789; -.
HGNC HGNC:2438; CSF3.
GenAtlas CSF3.
HPA CAB016131; -.
HPA001412; -.
MIM 138970; gene. [NCBI / EBI]
PharmGKB PA26941; -.
GeneCards P09919.
Gene expression databases
CleanEx HS_CSF3; -.
GermOnline ENSG00000108342; Homo sapiens.
Ontologies
GO
GO:0005615; Cellular component: extracellular space (traceable author statement from ProtInc).
GO:0019899; Molecular function: enzyme binding (inferred from physical interaction from UniProtKB).
GO:0005130; Molecular function: granulocyte colony-stimulating factor receptor binding (traceable author statement from ProtInc).
GO:0019221; Biological process: cytokine and chemokine mediated signaling pathway (non-traceable author statement from UniProtKB).
GO:0030851; Biological process: granulocyte differentiation (non-traceable author statement from UniProtKB).
GO:0008284; Biological process: positive regulation of cell proliferation (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR012351; 4_helix_cytokine_core.
IPR003629; GCSF_MGF.
IPR003573; IL6_MGF_GCSF.
Graphical view of domain structure.
Gene3D G3DSA:1.20.1250.10; 4_helix_cytokine_core; 1.
PANTHER PTHR10511:SF2; GCSF_MGF; 1.
Pfam PF00489; IL6; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF001935; IL6_MGF_GCSF; 1.
PRINTS PR00433; IL6GCSFMGF.
SMART SM00126; IL6; 1.
SMART graphical view of domain structure.
PROSITE PS00254; INTERLEUKIN_6; 1.
BLOCKS P09919.
Genome annotation databases
Ensembl ENSG00000108342; Homo sapiens. [Contig view]
GeneID 1440; -.
KEGG hsa:1440; -.
Phylogenomic databases
HOGENOM P09919; -.
HOVERGEN P09919; -.
Other
DrugBank DB00099; Filgrastim.
DB00019; Pegfilgrastim.
LinkHub P09919; -.
SOURCE CSF3; Homo sapiens.
ProtoNet P09919.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Alternative splicing; Cytokine; Direct protein sequencing; Glycoprotein; Growth factor; Pharmaceutical; Polymorphism; Secreted; Signal.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
SIGNAL   1    29  29     Probable. 
CHAIN   30   207  178     Granulocyte colony-stimulating factor. PRO_0000015570
CARBOHYD   166   166        O-linked (GalNAc...) (By similarity). 
DISULFID   69    75         
DISULFID   97   107         
VAR_SEQ   66    68        Missing (in isoform Short). VSP_002673
VARIANT   157   157  1     L -> M (in dbSNP:rs2227329 [NCBI]). VAR_013073 
VARIANT   174   174  1     A -> T (in dbSNP:rs2227330 [NCBI]). VAR_013074 
CONFLICT   30    30        A -> M (in Ref. 6; AA sequence). 
HELIX   41    71  31      
HELIX   77    80  4      
HELIX   81    86  6      
HELIX   105   124  20      
TURN   125   127  3      
TURN   130   132  3      
HELIX   133   156  24      
HELIX   176   204  29      
Sequence information
Length: 207 AA [This is the length of the unprocessed precursor] Molecular weight: 22293 Da [This is the MW of the unprocessed precursor] CRC64: 421F635ECC776996 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA 

        70         80         90        100        110        120 
LQEKLVSECA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ ALQLAGCLSQ LHSGLFLYQG 

       130        140        150        160        170        180 
LLQALEGISP ELGPTLDTLQ LDVADFATTI WQQMEELGMA PALQPTQGAM PAFASAFQRR 

       190        200 
AGGVLVASHL QSFLEVSYRV LRHLAQP 

P09919 in FASTA format

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