raxmlGUI 2.0 seamlessly integrates RAxML and RAxML-NG binaries for all major operating systems with an intuitive graphical front‐end to setup and run phylogenetic analyses. It offers automated pipelines for analyses that require multiple calls of RAxML, built‐in functions to concatenate alignment files, and one‐click model testing to select the best substitution models using ModelTest‐NG.